A simple method for the extraction and quality evaluation of genomic DNA

Abstract

In recent years, gene mutations (pathologic variant) have become a tool for diagnosing some diseases, even in fields such as pathology and hematology, and accordingly disease relatedgene testing (genetic testing and somatic mutation test) has become an indispensable diagnostic method. In cancer genome medicine, genomic DNA extracted from patient’s specimens are tested for them, making the extraction/purification of genomic DNA an indispensable technique. Thus, in this study, we established a simple and inexpensive method (salt method) for extracting genomic DNA, and compare its quality with that of a commercially available DNA extraction kit with multistep, silica adsorption column and special reagents. While the
commercial kits need highspeed centrifuge and special column and reagents for extraction, the salt method does not require those, and DNA can be extract using neutral detergent and cooking salt.
Compared with the commercial DNA extraction kit, the yield of the genomic DNA obtained by our simple method was 1.7fold higher. And more, examining the genomic DNA with electrophoresis and restriction enzyme digestion showed that the DNA extracted by our method is comparably intact. And, it is also inexpensive in terms of the cost. Although it is difficult to handle a large number of samples at the
same time, the reagents used in our method are commonly used in daily life and easy to obtain, making this technique useful in the education and unexpected situations with consumed reagent and kits.