Simple, Rapid Identification of Genetic Sex in Young Japanese Medaka, Oryzias latipes

Abstract

In genetic experiments designed for high school students, genetic privacy should be respected and human DNA samples should not be used. We previously developed experiments to determine the sex of medaka (Oryzias latipes) and then investigated PCR enzymes and extraction of DNA to devise simpler, more rapid experiments. A method using both KOD-FX for PCR enzymes and a BioMasher was subsequently developed. This method utilizes young fish in which sex is not distinguishable based on appearance.