Adsorption of Coomassi Brilliant Blue R-250 on the Rice Bran

Abstract

Coomassi Brilliant Blue R-250(CBB) has been used to stain proteins in the gel electrophoresis. In the gel electrophoresis, the destaining step is important to avoid the raise of background by dye and thereby, the fast and efficient gel destaining is needed. We studied whether the rice bran could adsorb CBB in the dye solution. As a result, higher adsorption of CBB on the rice bran was observed between pH 2.5 to 3.5. Moreover, adsorption of CBB increased with increasing the rice bran dosage and constant adsorption observed at dosage rate of 2.5 g/l and higher. On the other hand, adsorption of CBB on the rice bran was occurred rapidly in the early period and 75% of CBB was adsorbed in 1 min of agitation.  Our results showed that the rice bran could quickly adsorb CBB and was an effective adsorbent for CBB.